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Authors: Li Y, Wang XY, Zhang RZ, Zhang XY, Liu W, Xu XM, Zhang YW
Article Title: Molecular Imprinting and Immobilization of Cellulase Onto Magnetic Fe3O4@SiO2 Nanoparticles.
Publication date: 2014
Journal: Journal of Nanoscience and Nanotechnology
Volume: 14
Issue: (4)
Page numbers: 2931-2936.
DOI: 10.1166/jnn.2014.8625
Alternative URL: http://www.ingentaconnect.com/content/asp/jnn/2014/00000014/00000004/art00034
Abstract: Supermagnetic Fe3O4@SiO2 nanoparticles were molecular-imprinted prepared with cellulase as the template. The molecular imprinted nanoparticles were used as support to immobilization of cellulase. The transmission electron microscopy confirmed the core-shell structure and revealed that the size of the nanoparticles was around 10 nm. It was observed that cellulase was immobilized on the nanoparticles successfully from the Fourier transform infrared spectra. The adsorption of cellulase on the nanoparticles was specific and rapid. A high immobilization efficiency of 95% was achieved after the optimization. At 70 °C, the half-life of the immobilized cellulase was 3.3-fold of the free enzyme. Compared with the free enzyme, the immobilized cellulase has the same optimal pH, higher optimal temperature, better thermal stability and higher catalytic efficiency. The results strongly suggest that the immobilized cellulase on molecular imprinted Fe3O4@SiO2 has the potential applications in the production of bioethanol, paper and pulp industry, and pharmaceutical industry
Template and target information: protein, cellulase
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