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Abstract: A molecularly imprinted polymer-matrix solid-phase dispersion methodology for simultaneous determination of five steroids in goat milk samples was proposed. Factors affecting the extraction recovery such as sample/dispersant ratio and washing and elution solvents were investigated. The molecularly imprinted polymer used as dispersant in the matrix solid-phase dispersion procedure showed high affinity to steroids, and the obtained extracts were sufficiently clean to be directly analysed. Analytical separation was performed by micellar electrokinetic chromatography using a capillary electrophoresis system equipped with diode array detector. A background electrolyte composed by borate buffer (25 mM, pH 9.3), sodium dodecyl sulphate (10 mM) and acetonitrile (20%) was used. The developed MIP-MSPD methodology was applied to direct determination of testosterone (T), estrone (E1), 17β-estradiol (17β-E2), 17α-ethinylestradiol (EE2) and progesterone (P) in different goat milk samples. Mean recoveries obtained ranged from 81 to 110%, with relative standard deviations (RSD) £ 12%. The molecularly imprinted polymer-matrix solid-phase dispersion method is fast, selective, cost-effective and environmentally-friendly compared with other pretreatment methods used for extraction of steroids in milk
Template and target information: steroids, testosterone, T, estrone, E1, 17β-estradiol, 17β-E2, 17α-ethinylestradiol, EE2, progesterone, P
Author keywords: molecularly imprinted polymer, Matrix solid-phase dispersion, MEKC, steroids, Goat milk