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Abstract: Based on the incorporation of multi-walled carbon nanotubes (MWNTs) and molecular imprinting technique, an electrochemical sensor for the determination of clenbuterol was developed. The sensor was prepared by electropolymerizing o-phenylenediamine (o-PD) on the MWNTs modified glassy carbon electrode in the presence of the template clenbuterol. The template can be quickly removed in acetonitrile solution. Cyclic voltammetry (CV), AC impedance (IMP) and quartz crystal microbalance technique (QCM) were employed to characterize the performance of the modified electrode, the thickness of the membrance was determined to be 12.3 nm. The sensor provided an efficient way to eliminate interferences from the compounds with similar structures. A linear response between the relative peak current change of potassium hexacyanoferrate(III) and the concentration of clenbuterol was obtained in the range of 4.0 x 10-8 ~ 6.6 x 10-6 mol/L and the detection limit was 8.1 x 10-9 mol/L based on the 3σ of blank signals. The sensor was successfully applied to the determination of clenbuterol in pork with the recoveries from 101.3% to 107.9%.
Template and target information: clenbuterol
Author keywords: Multi-walled carbon nanotubes, molecular imprinting technique, clenbuterol, Electrochemical