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Abstract: Molecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g-1, excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R2 = 0.9970) and a favorable detectability up to 1.5 ng mL-1. Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research
Template and target information: protein, phycocyanin