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Reference type: Journal
Authors: Sellergren B
Article Title: Direct drug determination by selective sample enrichment on an imprinted polymer.
Publication date: 1994
Journal: Analytical Chemistry
Volume: 66
Issue: (9)
Page numbers: 1578-1582.
DOI: 10.1021/ac00081a036

Abstract: A new approach to sample enrichment and analyte determination is reported. An imprinted dispersion polymer capable of molecular recognition of pentamidine (PAM), a drug used for the treatment of AIDS-related pneumonia, was used in solid-phase extraction in order to selectively retain PAM from a dilute solution. At a physiological concentration (30 nM) this gave an enrichment factor of 54 using a PAM-selective polymer whereas the enrichment factor on a benzamidine- (BAM-) imprinted reference polymer was only 14. The high selectivity of the polymer allowed the drug to be detected directly in the desorption step, thus eliminating the need for a successive chromatographic analysis. In this way,; PAM could be enriched and directly analyzed when present in low concentration in a urine sample

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