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Abstract: In this work, we successfully developed bacterial templates on the surface of an overoxidized polypyrrole film using both gram-negative and gram-positive bacteria in which bacterial surface chemical structures are precisely transferred at a molecular level. The sensor film identified target bacteria within minutes through a unique combination with dielectrophoresis. The bacterial cavities had high selectivity for distinguishing specific target bacteria in bacterial mixtures containing gram-negative (Escherichia coli and Pseudomonas aeruginosa) and gram-positive (Bacillus subtilis and Staphylococcus aureus) bacteria. This rapid and specific recognition system will enable not only bacterial sensing but also analysis of various biological species
Template and target information: bacteria, gram-negative bacteria, Escherichia coli, Pseudomonas aeruginosa, gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus
Author keywords: conducting polymer, bacteria, detection, Molecularly imprinted polymers, Gram-negative bacteria, Gram-positive bacteria