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Reference type: Journal
Authors: Phan NVH, Sussitz HF, Lieberzeit PA
Article Title: Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP.
Publication date: 2014
Journal: Biosensors
Volume: 4
Issue: (2)
Page numbers: 161-171.
DOI: 10.3390/bios4020161
Alternative URL: http://www.mdpi.com/2079-6374/4/2/161/htm

Abstract: Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance - QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.
Template and target information: protein, bovine serum albumin, BSA
Author keywords: molecular imprinted polymer, protein imprinting, polymer optimization, QCM

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