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Abstract: A selective method for the determination of fourteen nitroimidazoles and their hydroxy-metabolites in honey was developed based on improved molecularly imprinted solid-phase extraction followed by liquid chromatography-tandem mass spectrometry. The separation of analytes was performed on a C18 column using a mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water with gradient elution. The method was suitable for metronidazole, hydroxymetronidazole, dimetridazole, ronidazole, hydroxydimetridazole, ipronidazole, hydroxyipronidazole, carnidazole, menidazole, nimorazole, ornidazole, secnidazole, ternidazole, and tinidazole. The procedure was evaluated according to EU Commission Decision 2002/657/EC requirements by determining linearity, specificity, recovery, repeatability, within-laboratory reproducibility, decision limit, detection capability, matrix effects, and stability. The method determined nitroimidazoles and their hydroxy-metabolites below the recommended concentration level of 3 μg kg-1. The decision limits and detection capabilities ranged from 0.110 μg kg-1 to 0.387 μg kg-1 and from 0.179 μg kg-1 to 0.508 μg kg-1, respectively. The results from stability tests indicated that all analyzed nitroimidazoles were stable in honey stored at 4-¦C for at least 28 weeks and that elevated temperature and exposure to light exposure accelerated their degradation. The method was successfully applied to the analysis of a wide variety of honey samples
Template and target information: nitroimidazoles, metronidazole, hydroxymetronidazole, dimetridazole, ronidazole, hydroxydimetridazole, ipronidazole, hydroxyipronidazole, carnidazole, menidazole, nimorazole, ornidazole, secnidazole, ternidazole, tinidazole
Author keywords: Honey, HPLC, liquid chromatography tandem mass spectrometry, molecularly imprinted solid-phase extraction, nitroimidazoles