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Abstract: A series of imprinted polymers targeting nucleoside metabolites, prepared using a template analogue approach, are presented. These were prepared following selection of the optimum functional monomer by solution association studies using 1H NMR titrations whereby methacrylic acid was shown to be the strongest receptor with and affinity constant of 621 -¦ 51 L mol-1 vs. 110 -¦ 16 L mol-1 for acrylamide. The best performing polymers were prepared using methanol as porogenic co-solvent and although average binding site affinities were marginally reduced, 2.3 x 10^4 L mol-1 vs. 2.7 x 10^4 L mol-1 measured for a polymer prepared in acetonitrile, these polymers contained the highest number of binding sites, 5.27 μmol g-1 vs. 1.64 μmol g-1, while they also exhibited enhanced selectivity for methylated guanosine derivatives. When applied as sorbents in the extraction of nucleoside derivative cancer biomarkers from synthetic urine samples, significant sample clean-up and recoveries of up to 90% for 7-methylguanosine were achieved
Template and target information: nucleoside metabolites, 7-methylguanosine
Author keywords: molecular imprinting, Nucleosides, 7-Methyl guanosine, bioanalysis, solid phase extraction (SPE)