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Abstract: A bovine serum albumin (BSA) imprinted nonwoven polypropylene (PP) fibre supported calcium alginate/polyacrylamide (CA/PAM) hydrogel film (PP-s-CA/PAM MIP) was prepared using nonwoven PP as matrix, BSA as template molecule, sodium alginate (SA) and acrylamide (AM) as functional monomers, N,N'-methylenebisacrylamide (MBA) as covalent cross-linker, and CaCl2 as ionic cross-linker by UV radiation-reduced polymerization in an aqueous phase. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted PP-s-CA/PAM MIPs were investigated. The results showed that PP-s-CA/PAM MIPs exhibited an obvious improvement in terms of adsorption capacity and recognition for BSA compared with the non-imprinted ones. The adsorption capacity of MIP for BSA reached 27.02 mg g-1 when the adsorption time was 900 min, which was 2.8 times the non-imprinted one. The swelling of the CA/PAM hydrogel supported on PP was significantly reduced. In addition, the regeneration property of the BSA-imprinted PP-s-CA/PAM hydrogel was distinctly improved. The imprinting efficiency of PP-s-CA/PAM MIP maintained 92.95% of the initial value even after five repetitions
Template and target information: protein, bovine serum albumin, BSA