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Abstract: Growing public concerns about biosafety make continuous monitoring of biospecies increasingly interesting. Escherichia coli (E. coli) is both an interesting analyte and a comparably non-pathogenic model organism for developing novel sensors suitable for that purpose. For such sensors, molecularly imprinted polymers (MIP) are a promising approach to create selective, sensitive and reversible recognition layers. This work presents a biosensor based on imprinted polyurethane and characterizes it in terms of selectivity and sensitivity towards b-strain E. coli. Atomic force microscopy (AFM) revealed successful imprinting and uptake of the bacteria into the polymer. On quartz crystal microbalance (QCM), such MIP yield linear sensor characteristics with a detection limit of 1.6 x 10^8 cells/ml. The sensor shows a selectivity factor of five compared to smaller w-strain E. coli. To assess the capabilities of such sensors for on-line monitoring, we designed a small model bioreactor (50 ml) and followed growth processes as a proof of principle. The different phases of bacteria growth can be observed in real time via the frequency responses on the MIP. Sensor responses could be validated by cell counting
Template and target information: bacteria, Escherichia coli, E. coli
Author keywords: bio monitoring, biosensor, Escherichia coli, molecularly imprinted polymer, real-time measurement