Book title: Affinity Chromatography
Edition: 1286
Editors: Reichelt S
Publisher: Springer
City: New York
ISBN: 978-1-4939-2446-2
Series title: Methods in Molecular Biology
Abstract: Molecularly imprinted polymers can be used for the selective capture of a target molecule from complex medium. Cryogels novel matrices, which characterized by their supermacropores that makes their use advantageous when studying with biological samples. By combining high selectivity of the molecular imprinting approach with using cryogel as a base polymer, in this protocol, preparation of the albumin-imprinted cryogels is described. This material is a useful candidate for the selective albumin depletion from the human serum sample prior to the detailed proteomic analysis
Template and target information: protein, albumin, human serum albumin, HSA
Author keywords: Albumin depletion, molecular imprinting, protein imprinting, Cryogel