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Reference type: Journal
Authors: Wang CL, Hu XL, Guan P, Wu DF, Qian LW, Li J, Song RY
Article Title: Lysozyme Molecular Imprinted Membranes with Ionic Liquids as Functional Monomer.
Publication date: 2015
Journal: Acta Polymerica Sinica
Issue: (3)
Page numbers: 259-265.
DOI: 10.11777/j.issn1000-3304.2015.14224
Alternative URL: http://www.gfzxb.org/EN/abstract/abstract14285.shtml

Abstract: In order to increase structural selectivity,surface-initiated ATRP and ionic liquid (1-vinyl-3-ethyl acetate imidazolium chloride) as functional monomer were used to prepare molecularly imprinting membranes(MIMs).MIMs were prepared with lysozyme as template,ionic liquids (1-vinyl-3-ethyl acetate imidazolium chloride) as functional monomer,MBA as cross-linking agent,PMDETA as ligand,CuCl as catalyst through surface7-initiated ATRP on the initiator-functional regenerated cellulose membranes in aqueous solution at 35C.The imprinted density and thickness of lysozyme molecular imprinted membranes were controlled by varying the concentrations of functional monomer.The imprinting factor α reaches the maximum (6.25) at the ratio of functional monomer to initiator 100:1,with the water flux 83.0 L/m2/h.The equilibrium experimental data for the lysozyme-MIMs fitted the Langmuir isotherm well,and the binding amount reached 14.8 mg/g.The solid phase extraction experiments demonstrated that lysozyme-MIMs had specific separation and recognition performance in 4 min.Furthermore,permeability analysis experiments demonstrated that the lysozyme-MIMs had higher penetration ability for the target molecules than competitive molecules Cyt C and BHb.Based on the data obtained in separation of lysozyme from the mixed solution,the lysozyme-MIMs separation mechanism belongs to facilitated permeation,the template molecular lysozyme is first specifically adsorbed and concentrated,and then moves through the imprinted membrane by the binding/desorption process.
Template and target information: protein, lysozyme
Author keywords: Ionic liquid, molecularly imprinting, ATRP, Regenerated cellulose membranes, lysozyme

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