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Reference type: Journal
Authors: Scorrano S, Mergola L, Di Bello MP, Lazzoi MR, Vasapollo G, del Sole R
Article Title: Molecularly Imprinted Composite Membranes for Selective Detection of 2-Deoxyadenosine in Urine Samples.
Publication date: 2015
Journal: International Journal of Molecular Sciences
Volume: 16
Issue: (6)
Page numbers: 13746-13759.
DOI: 10.3390/ijms160613746

Abstract: An important challenge for scientific research is the production of artificial systems able to mimic the recognition mechanisms occurring at the molecular level in living systems. A valid contribution in this direction resulted from the development of molecular imprinting. In this work, a novel molecularly imprinted polymer composite membrane (MIM) was synthesized and employed for the selective detection in urine samples of 2-deoxyadenosine (2-dA), an important tumoral marker. By thermal polymerization, the 2-dA-MIM was cross-linked on the surface of a polyvinylidene-difluoride (PVDF) membrane. By characterization techniques, the linking of the imprinted polymer on the surface of the membrane was found. Batch-wise guest binding experiments confirmed the absorption capacity of the synthesized membrane towards the template molecule. Subsequently, a time-course of 2-dA retention on membrane was performed and the best minimum time (30 min) to bind the molecule was established. HPLC analysis was also performed to carry out a rapid detection of target molecule in urine sample with a recovery capacity of 85%. The experiments indicated that the MIM was highly selective and can be used for revealing the presence of 2-dA in urine samples.
Template and target information: 2-deoxyadenosine, 2-dA
Author keywords: 2-deoxyadenosine, Molecularly Imprinted Membrane (MIM), urine detection, selective separation

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