Abstract: Molecular imprinted titania nanoparticles were developed for selective recognition of purine base e.g. guanine and their final oxidation product. Titania nanoparticles were prepared by hydrolysis of titanium butoxide as precursor in the presence of pattern molecules. The morphology of synthesized nanoparticles is evaluated by SEM images. Recognition characteristics of imprinted titania nanoparticles was studied by exposing them to standard solution of guanine and uric acid respectively. The resultant change in their concentration is determined by UV/Vis analysis that indicated imprinted titania nanoparticles possess high affinity for print molecules. In both cases, non-imprinted titania was taken as control to observe non-specific binding interactions. Cross sensitivity studies suggested that imprinted titania is at least five times more selective for binding print molecules than competing analytes solution thus, indicating its potential for bioassay of purines and their oxidation products.
Template and target information: purines, guanine
Author keywords: molecular imprinting, Guanine, uric acid, titania, nanoparticles