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Reference type: Journal
Authors: Mukherjee J, Gupta MN
Article Title: Molecular bioimprinting of lipases with surfactants and its functional consequences in low water media.
Publication date: 2015
Journal: International Journal of Biological Macromolecules
Volume: 81
Page numbers: 544-551.
DOI: 10.1016/j.ijbiomac.2015.08.033
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0141813015005772

Abstract: Lipases from Thermomyces lanuginosa (TLL), Candida rugosa (CRL) and Burkholderia cepacia (BCL) were obtained in the 'open lid' form by adding surfactant molecules like n-octyl-β-d-glucopyranoside (OG), hexadecyl trimethyl ammonium bromide (CTAB), Bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) and triton X-100 for this purpose. The enzymes were 'dried' by precipitating with 4x (v/v) excess of organic solvents. The imprint surfactant molecules were removed by extensive washing with organic solvents. TLL imprinted with 0.05% CTAB showed 11-fold increase in the transesterification activity and was a better preparation to kinetically resolve (-)-1-phenylethanol. Fluorescence emission spectra confirmed that Trp89 of the lid was indeed affected during bioimprinting. With CRL, bioimprinting with OG gave 7-fold increase in the transesterification rates and resulted in reversal of enantioselectivity of CRL and gave R-phenylethyl acetate instead of the S-product as with the unimprinted precipitate. Bioimprinted BCL was also a 7-fold better catalyst for transesterification as well as enantioselectivity
Template and target information: bioimprinting
Author keywords: Enzymes in organic solvents, kinetic resolution, lipases, bioimprinting, Enzyme precipitated and rinsed with organic solvents (EPROS)


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