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Reference type: Journal
Authors: Cenci L, Andreetto E, Vestri A, Bovi M, Barozzi M, Iacob E, Busato M, Castagna A, Girelli D, Bossi AM
Article Title: Surface plasmon resonance based on molecularly imprinted nanoparticles for the picomolar detection of the iron regulating hormone Hepcidin-25.
Publication date: 2015
Journal: Journal of Nanobiotechnology
Volume: 13
Page numbers: ArticleNo51.
DOI: 10.1186/s12951-015-0115-3
Alternative URL: http://www.jnanobiotechnology.com/content/13/1/51

Abstract: Background Molecularly imprinted polymer (MIP) technique is a powerful mean to produce tailor made synthetic recognition sites. Here precipitation polymerization was exploited to produce a library of MIP nanoparticles (NPs) targeting the N terminus of the hormone Hepcidin-25, whose serum levels correlate with iron dis-metabolisms and doping. Biotinylated MIP NPs were immobilized to NeutrAvidin™ SPR sensor chip. The response of the MIP NP sensor to Hepcidin-25 was studied. Findings Morphological analysis showed MIP NPs of 20-50 nm; MIP NP exhibited high affinity and selectivity for the target analyte: low nanomolar Kds for the interaction NP/Hepcidin-25, but none for the NP/non regulative Hepcidin-20. The MIP NP were integrated as recognition element in SPR allowing the detection of Hepcidin-25 in 3 min. Linearity was observed with the logarithm of Hepcidin-25 concentration in the range 7.2-720 pM. LOD was 5 pM. The response for Hepcidin-20 was limited. Hepcidin-25 determination in real serum samples spiked with known analyte concentrations was also attempted. Conclusion The integration of MIP NP to SPR allowed the determination of Hepcidin-25 at picomolar concentrations in short times outperforming the actual state of art. Optimization is still needed for real sample measurements in view of future clinical applications.
Template and target information: peptide, hepcidin-25
Author keywords: Molecularly imprinted polymers, nanoparticles, Hepcidin, biosensor, surface plasmon resonance, Iron metabolism


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