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Reference type: Journal
Authors: Yang CC, Yan XM, Guo H, Fu GQ
Article Title: Synthesis of surface protein-imprinted nanoparticles endowed with reversible physical cross-links.
Publication date: 2016
Journal: Biosensors and Bioelectronics
Volume: 75
Page numbers: 129-135.
DOI: 10.1016/j.bios.2015.08.033
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566315303572

Abstract: Researches on protein molecularly imprinted polymers have been challenged by the difficulties in facilitating biomacromolecular transfer, in particular upon the template removal step, and enhancing their recognition performance. Addressing these issues, herein we report synthesis of coreGă˘shell structured surface protein-imprinted nanoparticles with reversible physical cross-links formed in the imprinted nanoshells. The imprinted layers over nanoparticle supports are fabricated via aqueous precipitation polymerization (PP) of di(ethylene glycol) methyl ether methacrylate (MEO2MA), a thermo-responsive monomer bearing no strong H-bond donor, and other functional and cross-linking monomers. During polymerization, physical cross-links together with chemical cross-links are in site produced within the imprinted shells based on hydrophobic association among the PMEO2MA, favoring formation of high-quality imprints. While cooled appropriately below the polymerization temperature, these physical cross-links can be dissociated rapidly, thus facilitating removal of the embedded template. For proof of this concept, lysozyme-imprinted nanoparticles were synthesized at 37 ░C over the nanoparticles functionalized with carboxylic and vinyl groups. The template removal from the imprinted nanoparticles was readily achieved by washing with a dilute acidic detergent solution at 4 ░C. As-prepared imprinted nanoparticles showed greatly higher imprinting factor and specific rebinding than obtained with the same recipe but by solution polymerization (SP). Moreover, such imprinted nanomaterials exhibited satisfactory rebinding selectivity, kinetics and reusability
Template and target information: protein, lysozyme
Author keywords: protein imprinting, surface imprinting, nanoparticles, Reversible physical cross-links, Thermo-responsive polymers, Aqueous precipitation polymerization

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