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Reference type: Journal
Authors: Xiao TT, Shi XZ, Jiao HF, Sun AL, Ding H, Zhang RR, Pan DD, Li DX, Chen J
Article Title: Selective and sensitive determination of cypermethrin in fish via enzyme-linked immunosorbent assay-like method based on molecularly imprinted artificial antibody-quantum dot optosensing materials.
Publication date: 2016
Journal: Biosensors and Bioelectronics
Volume: 75
Page numbers: 34-40.
DOI: 10.1016/j.bios.2015.08.014

Abstract: Molecularly imprinted silica layers appended to quantum dots (MIP-QDs) with customized selective artificial recognition sites were fabricated in this study by optimizing the ratio of the functional monomer to the template. Scanning electron microscopy, transmission electron microscopy, Brunauer-Emmett-Teller, Fourier transform infrared spectroscopy, and selectivity assay analyses were also performed. Results demonstrated that the selective fluorescence quenching properties of MIP-QDs toward cypermethrin (CYP) are due to strong interactions between these molecules. An enzyme-linked immunosorbent assay (ELISA)-like method based on the MIP-QDs was established under optimal conditions. The fluorescence quenching observed from this method showed a linear relationship with CYP concentration over the range of 0.05-60.0 mg/kg with a correlation coefficient of 0.9838. Good recovery (82.7-92.4%) and a relative standard deviation of less than 10.1% were obtained from fish samples spiked with three levels of CYP. This method also demonstrated a low detection limit of 1.2 μg/kg. The ELISA-like method based on MIP-QDs can be successfully employed to detect residual of CYP in fish samples
Template and target information: cypermethrin, CYP
Author keywords: Pyrethroid, molecularly imprinted, quantum dot, Optosensing materials


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