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Reference type: Journal
Authors: Wang QL, Chen MM, Zhang HQ, Wen W, Zhang XH, Wang SF
Article Title: Solid-state electrochemiluminescence sensor based on RuSi nanoparticles combined with molecularly imprinted polymer for the determination of ochratoxin A.
Publication date: 2016
Journal: Sensors and Actuators B: Chemical
Volume: 222
Page numbers: 264-269.
DOI: 10.1016/j.snb.2015.08.057

Abstract: An electrochemiluminescence (ECL) sensor based on Ru(bpy)32+-doped silica nanoparticles (RuSi NPs) combined with molecularly imprinted polymer (MIP) has been developed for the determination of ochratoxin A (OTA). The sensor was fabricated by remodification of RuSi NPs with a thin film of molecularly imprinted polymer, which provided the specific binding sites for OTA. The process of template elution and rebinding acted as a gate to control the flux of probes, which passed through the cavities and reacted with Ru(bpy)32+ immobilized on the electrode surface to emit ECL signal. The ECL intensity decreased with the OTA molecules rebound in MIP. The as-prepared sensor exhibited a very high sensitivity and excellent selectivity to the target molecule. The Δ IECL depended linearly on the logarithm of the OTA concentration in the range from 0.1 pg/mL to 14.76 ng/mL with lower detection limit of 0.027 pg/mL (S/N = 3). The MIP-ECL sensor enabled the successful application of this method to determine OTA in corn
Template and target information: ochratoxin A, OTA
Author keywords: Electrochemiluminescence sensor, Ru-silica nanoparticles, Molecularly imprinted polymers, ochratoxin A

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