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Reference type: Journal
Authors: de Lima MM, Vieira AC, Martins I, Boralli VB, Borges KB, Figueiredo EC
Article Title: On-line restricted access molecularly imprinted solid phase extraction of ivermectin in meat samples followed by HPLC-UV analysis.
Publication date: 2016
Journal: Food Chemistry
Volume: 197
Issue: (Part A)
Page numbers: 7-13.
DOI: 10.1016/j.foodchem.2015.10.082
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814615300765

Abstract: A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was synthesized, characterized and used for direct analysis of ivermectin from bovine meat samples, in a two-dimensional liquid chromatography system with UV detection. Ivermectin, 4-vinylpiridine and ethylene glycol dimethacrylate were employed as template, functional monomer and cross-linker, respectively. A BSA layer was cross-linked around the polymer, resulting in a biocompatible chemical barrier able to eliminate about 100% of protein from the samples. Ivermectin was extracted from the minced meat samples through a solvent extraction using methanol:water (70:30, v:v), and the extracts were directly injected into the two-dimensional liquid chromatography system, without any other treatment. Samples, fortified with ivermectin from 50 to 500 μg kg-1, were used to build the analytical calibration curve (r = 0.996). The limit of quantification was 50 μg kg-1. Precision and accuracy presented variation coefficients, as well as relative errors lower than 17.0% and within -18.5% and 22.0%, respectively
Template and target information: ivermectin
Author keywords: Restricted access molecularly imprinted polymer, BSA layer, RAMIP-BSA, Meat, Ivermectin


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