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Reference type: Journal
Authors: Guo JX, Wang YZ, Liu YJ, Zhou YG
Article Title: The synthesis of imprinted polymers based on Fe3O4 nanomaterials and the recognition of proteins.
Publication date: 2015
Journal: Analytical Methods
Volume: 7
Issue: (23)
Page numbers: 10018-10025.
DOI: 10.1039/C5AY02510A

Abstract: A surface molecularly imprinted polymer (MIP-Fe3O4@AA) was prepared on a magnetic material (Fe3O4@AA) surface for the selective recognition and adsorption of bovine hemoglobin (BHb). The acrylic acid (AA) monomer was directly grafted to the surface of Fe3O4 through a simple coordination reaction between the carboxyl group of AA and unsaturated iron ions (Fe3O4@AA). Then the composite of MIP-Fe3O4@AA based on BHb was successfully synthesized by the surface grafting copolymerization method. The amounts of raw materials were optimized. The characteristics of the obtained MIP-Fe3O4@AA were determined by Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), vibrating sample magnetometry (VSM) and thermogravimetric analysis (TGA). The adsorption capacity of MIP-Fe3O4@AA was evaluated and well fitted by the Langmuir model. The maximum theoretical adsorption capacity (Qmax) was calculated to be 117.27 mg g-1 for MIP-Fe3O4@AA. The selectivity experiment showed that the MIP-Fe3O4@AA had an outstanding selectivity toward the template protein (BHb) with an imprinting factor of 4.43. The MIP-Fe3O4@AA was also used to capture BHb from the protein mixture and real sample with satisfactory results, which demonstrated its potential practical analytical performance
Template and target information: protein, bovine hemoglobin, BHb


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