Reference type: Journal
Authors: Bousoumah R, Antignac JP, Camel V, Grimaldi M, Balaguer P, Courant F, Bichon E, Morvan ML, Le Bizec B
Article Title: Development of a molecular recognition based approach for multi-residue extraction of estrogenic endocrine disruptors from biological fluids coupled to liquid chromatography-tandem mass spectrometry measurement.
Publication date: 2015
Journal: Analytical and Bioanalytical Chemistry
Volume: 407
Issue: (29)
Page numbers: 8713-8723.
DOI: 10.1007/s00216-015-9024-4

Abstract: Multi-residue methods permitting the high-throughput and affordable simultaneous determination of an extended range of endocrine disrupting chemicals (EDCs) with reduced time and cost of analysis is of prime interest in order to characterize a whole set of bioactive compounds. Such a method based on UHPLC-MS/MS measurement and dedicated to 13 estrogenic EDCs was developed and applied to biological matrices. Two molecular recognition-based strategies, either molecular imprinted polymer (MIP) with phenolic template or estrogen receptors (ERα) immobilized on a sorbent, were assessed in terms of recovery and purification efficiency. Both approaches demonstrated their suitability to measure ultra-trace levels of estrogenic EDCs in aqueous samples. Applicability of the MIP procedure to urine and serum samples has also been demonstrated
Template and target information: estrogenic compounds, commercial MIP
Author keywords: Estrogenic endocrine disrupting chemicals, molecular imprinted polymer, Estrogen receptors, Biological matrices, UHPLC-MS, MS