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Reference type: Journal
Authors: Li DY, Zhang XM, Yan YJ, He XW, Li WY, Zhang YK
Article Title: Thermo-sensitive imprinted polymer embedded carbon dots using epitope approach.
Publication date: 2016
Journal: Biosensors and Bioelectronics
Volume: 79
Page numbers: 187-192.
DOI: 10.1016/j.bios.2015.12.016
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566315306710

Abstract: A new type of thermo-sensitive receptor carbon dots/SiO2/molecularly imprinted polymer (CDs/SiO2/MIP) was prepared by surface imprinting procedure and the epitope approach. The synthetic CDs/SiO2/MIP was able to selectively capture target protein with fluorescence quenching via the special interaction between them and the recognition cavities. The receptor exhibited the linear fluorescence quenching to cytochrome c (cyt c) in the range of 0.1-40 μM, and the detection limit was 89 nM. The precision for five replicate detection of cyt c at 20 μM was 3.11%. Moreover, the receptor owned the temperature-sensitive element that allowed for swelling and shrinking in response to temperature changes to realize recognition of the target cytochrome c. The proposed strategy revealed the feasibility of fabrication of a thermo-sensitive imprinted polymer based on CDs and surface imprinting procedure and the epitope approach
Template and target information: protein, epitope, cytochrome ccyt c
Author keywords: molecularly imprinted polymer, Carbon dots, epitope approach, Fluorescent recognition


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