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Reference type: Journal
Authors: Gao RX, Cui XH, Hao Y, He GY, Zhang M, Tang YH
Article Title: Preparation of Cu2+-mediated magnetic imprinted polymers for the selective sorption of bovine hemoglobin.
Publication date: 2016
Journal: Talanta
Volume: 150
Page numbers: 46-53.
DOI: 10.1016/j.talanta.2015.12.017
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914015305476

Abstract: In this work, a novel Cu2+-mediated core-shell bovine hemoglobin imprinted superparamagnetic polymers were synthesized. First, carboxyl group directly-functionalized Fe3O4 nanoparticles were produced by a facile one-pot hydrothermal method. Next, copper ions were introduced to chelate with carboxyl groups and further bonded with template bovine hemoglobin as co-functional monomer. Then, functional monomers 3-aminopropyltriethoxylsilane and octyltrimethoxysilane were adopted to form the thin polymer layers. Finally, after removal of the templates, the imprinting shells with specific recognition cavities for bovine hemoglobin were obtained on Fe3O4 nanoparticles. The resultant molecularly imprinted polymers have high adsorption capacity and satisfactory selectivity for bovine hemoglobin with the help of copper ions. The obtained magnetic nanomaterials were characterized by transmission electron microscopy, Fourier-transform infrared spectra, X-ray diffraction, and vibrating sample magnetometer. The measurements demonstrated that the as-synthesized nanomaterials exhibited good dispersion, high crystallinity, and satisfactory superparamagnetic properties. The feasibility of this method was further confirmed by using the imprinted nanomaterials to specifically extract bovine hemoglobin from real bovine blood samples
Template and target information: protein, bovine hemoglobin
Author keywords: Molecularly imprinted polymers, magnetic separation, copper ions


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