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Reference type: Journal
Authors: Yang XQ, Xia Y
Article Title: Selective enrichment and separation of phosphotyrosine peptides by thermosensitive molecularly imprinted polymers.
Publication date: 2016
Journal: Journal of Separation Science
Volume: 39
Issue: (2)
Page numbers: 419-426.
DOI: 10.1002/jssc.201501063

Abstract: Novel thermosensitive molecularly imprinted polymers were successfully prepared using the epitope imprinting approach in the presence of the mimic template phenylphosphonic acid, the functional monomer vinylphosphonic acid-Ti4+, the temperature-sensitive monomer N-isopropylacrylamide and the crosslinker N,N'-methylenebisacrylamide. The ratio of the template/thermosensitive monomers/crosslinker was optimized, and when the ratio was 2:2:1, the prepared thermosensitive molecularly imprinted polymers had the highest imprinting factor. The synthetic thermosensitive molecularly imprinted polymers were characterized by Fourier transform infrared spectroscopy to reveal the combination and elution processes of the template. Then, the adsorption capacity and thermosensitivity was measured. When the temperature was 28 C, the imprinting factor was the highest. The selectivity and adsorption capacity of the thermosensitive molecularly imprinted polymers for phosphotyrosine peptides from a mixture of three tailor-made peptides were measured by high-performance liquid chromatography. The results showed that the thermosensitive molecularly imprinted polymers have good selectivity for phosphotyrosine peptides. Finally, the imprinted hydrogels were applied to specifically adsorb phosphotyrosine peptides from a sample mixture containing phosphotyrosine and a tryptic digest of β-casein, which demonstrated high selectivity. After four rebinding cycles, 78.9% adsorption efficiency was still retained
Template and target information: mimic template, phenylphosphonic acid, phosphotyrosine peptides, epitope
Author keywords: Epitope imprinting, Molecularly imprinted polymers, Phosphotyrosine, Thermosensitive hydrogels


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