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Abstract: In this paper, a new chemiluminescence (CL) sensor possessing high selectivity and sensitivity was established for determination of lysozyme using magnetic graphene oxide-multi-walled carbon nanotube surface molecularly imprinted polymer (MGO-MWCNTs/SMIP). The MGO-MWCNTs/SMIP was characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM), and the maximum adsorption capacity of MGO-MWCNTs/SMIP to lysozyme was found to be 140 mg g-1. The MGO-MWCNTs/SMIP was fixed into a glass tube and was connected to a chemiluminescent analyzer. Then a MGO-MWCNTs/SMIP-flow injection chemiluminescence (MGO-MWCNTs/SMIP-CL) sensor based on a luminol-NaOH-H2O2 CL system was established for the determination of lysozyme. The proposed sensor with high selectivity and sensitivity responded linearly to the concentration of lysozyme over the range 5.04 x 10-9 to 4.27 x 10-7 g mL-1 and the detection limit was 1.90 x 10-9 g mL-1 (3δ ). The recoveries ranged from 98% to 111% when determining lysozyme in eggs and the result was satisfactory. The advantageous properties of sensor hold the potential to be applied in protein analysis, analogizing to biological analysis
Template and target information: protein, lysozyme