Abstract: Quercetin imprinted polymer was synthesized by a reversible addition-fragmentation chain transfer(RAFT) polymerization method, combining molecular imprinting with living radical polymerization techniques, using the dibenzyltrithiocarbonate(DBTTC) as reversible RAFT reagent, quercetin as the imprinted compound, and the methacrylic acid(MAA), acrylamide(AM), acrylic acid(AA), 4-vinylpyridine(4-VP) and 2-vinylpyridine(2-VP) as functional monomers, ethyleneglycol dimethacrylate(EDMA) as cross-linker and 2,2-azobisisobutyronitrile(AIBN) as initiator.The preparation of molecular imprinted polymers(MIPs) were conducted under different experimental conditions, and the structures of the MIPs were characterized by nitrogen adsorption and scanning electron microscopy, respectively. The identify ability and separation efficiency of MIPs for quercetin were evaluated by high performance liquid chromatography(HPLC). The relationship among the polymerization conditions, structure of polymer and separation efficiency was studied, and the synthetic method and characteristics of MIPs prepared by living radical polymerization were also explored. The quercetin was separated and enriched in Zukamu Granules for Uighur medicine, using synthesized MIPs as stationary phase. The results showed that the MIPs synthesized by living radical polymerization method exhibited a better morphology, and a good adsorption efficiency for the target molecule.
Template and target information: quercetin
Author keywords: living radical polymerization, molecular imprinted polymer, quercetin, Zukamu granules for Uighur medicine, high performance liquid chromatography