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Reference type: Journal
Authors: Yoshizawa S, Kuwata T, Takano E, Kitayama Y, Takeuchi T
Article Title: Transcription-Type Protein Imprinted Polymers for SPR Sensing Prepared Using Target-immobilized Stamps based on Submicrometer-Sized Particles via Biotin-Avidin Linkage.
Publication date: 2015
Journal: Molecular Imprinting
Volume: 3
Issue: (1)
Page numbers: 26-34.
DOI: 10.1515/molim-2015-0007
Alternative URL: http://www.degruyter.com/view/j/molim.2015.3.issue-1/molim-2015-0007/molim-2015-0007.xml?format=INT

Abstract: Transcribed molecularly imprinted polymers (MIPs), prepared by using the biotinylated submicrometersized particles-immobilized stamp on which the biotinconjugated protein was hierarchically immobilized via avidin, were used successfully in the recognition of cytochrome c (Cyt) as a model protein. The transcribed MIP for Cyt was prepared on the gold-coated surface plasmon resonance (SPR) sensor chip, and the binding behavior of Cyt, myoglobin, ribonuclease A, lysozyme, and avidin was evaluated to confirm the selectivity for Cyt. The imprint effect of the transcribed MIP was revealed by comparing the MIP and the corresponding non-imprinted polymer prepared using the stamp without the Cyt immobilization.
Template and target information: protein, cytochrome c, Cyt
Author keywords: molecularly imprinted polymer, Transcription type molecular imprinting, protein, surface plasmon resonance, molecular recognition


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