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Reference type: Journal
Authors: Liu YB, Zhai JQ, Dong JT, Zhao MP
Article Title: Magnetic surface imprinted hydrogel nanoparticles for specific and reversible stabilization of proteins.
Publication date: 2015
Journal: Molecular Imprinting
Volume: 3
Issue: (1)
Page numbers: 47-54.
DOI: 10.1515/molim-2015-0006
Alternative URL: http://www.degruyter.com/view/j/molim.2014.2.issue-1/molim-2015-0006/molim-2015-0006.xml?format=INT

Abstract: In this work, we demonstrate the unique capability of surface imprinted hydrogels over magnetic nanoparticles (Fe3O4@MIP-NPs) for protection of enzyme proteins from denaturation induced by heat, UV irradiation, ultrasonication and addition of urea. Moreover, the Fe3O4@MIP-NPs successfully enabled rapid renaturation of heat-denatured proteins under mild conditions. These interesting properties of MIP nanoparticles offer useful tools for protection and renaturation of proteins in various applications.
Template and target information: protein, deoxyribonuclease I, DNase I
Author keywords: Surface imprinted hydrogel, magnetic nanoparticles, Selective binding, Protein protection, renaturation

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