MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Naklua W, Suedee R, Lieberzeit PA
Article Title: Dopaminergic receptor-ligand binding assays based on molecularly imprinted polymers on quartz crystal microbalance sensors.
Publication date: 2016
Journal: Biosensors and Bioelectronics
Volume: 81
Page numbers: 117-124.
DOI: 10.1016/j.bios.2016.02.047
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566316301543

Abstract: Molecularly imprinted polymers (MIPs) have been successfully applied as selective materials for assessing the binding activity of agonist and antagonist of dopamine D1 receptor (D1R) by using quartz crystal microbalance (QCM). In this study, D1R derived from rat hypothalamus was used as a template and thus self-organized on stamps. Those were pressed into an oligomer film consisting of acrylic acid: N-vinylpyrrolidone: N,N'-(1,2-dihydroxyethylene) bis-acrylamide in a ratio of 2:3:12 spin coated onto a dual electrode QCM. Such we obtained one D1R-MIP-QCM electrode, whereas the other electrode carried the non-imprinted control polymer (NIP) that had remained untreated. Successful imprinting of D1R was confirmed by AFM. The polymer can re-incorporate D1R leading to frequency responses of 100-1200 Hz in a concentration range of 5.9-47.2 -ÁM. In a further step such frequency changes proved inherently useful for examining the binding properties of test ligands to D1R. The resulting mass-sensitive measurements revealed Kd of dopamineGêÖHCl, haloperidol, and (+)-SCH23390 at 0.874, 25.6, and 0.004 nM, respectively. These results correlate well with the values determined in radio ligand binding assays. Our experiments revealed that D1R-MIP sensors are useful for estimating the strength of ligand binding to the active single site. Therefore, we have developed a biomimetic surface imprinting strategy for QCM studies of D1R-ligand binding and presented a new method to ligand binding assay for D1R
Template and target information: dopamine D1 receptor, D1R
Author keywords: molecularly imprinted polymer, Dopamine receptor, quartz crystal microbalance, Ligand binding assay


  mipdatabase.com logo special offer joke shirt  Mug featuring the name Peter spelled out in the single letter amino acid code  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner