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Reference type: Journal
Authors: Rong F, Wang H, Zhao YJ, Feng XG, Deng JJ
Article Title: Molecularly imprinted photonic crystal microspheres for label-free detection.
Publication date: 2016
Journal: Nanomedicine: Nanotechnology, Biology and Medicine
Volume: 12
Issue: (2)
Page numbers: 524.
DOI: 10.1016/j.nano.2015.12.219
Alternative URL: http://www.sciencedirect.com/science/article/pii/S154996341500444X

Abstract: In this paper, we propose a new label-free detecting technology that is combined with photonic crystal microspheres and molecular imprinting materials with the ability of biomimetic recognition. Firstly, the photonic microsphere used as the template of inverse opal structure was fabricated by self-assembling of silica nanospheres. Then, the polymerization in the void of photonic crystal microsphere template was performed. In this imprinted polymerization system, phenylboronic acid was chosen as monomers, PEGDA as cross-linkers and glucose as molecularly imprinted template molecules, respectively. After removal of photonic crystal template and the molecularly imprinted template, the molecularly imprinted photonic crystal microsphere (MIPCM) with a three-dimensional ordered inverse opal structure was generated, and the imprinted cavities left in the structure had specific recognition ability to template molecules during imprinting. The MIPCM can interact with the glucose in aqueous solution in alkaline conditions leading to a specific change of the diffraction peak of MIPCM. When 0.1 M NaCl solution was added, the glucose molecules could dissociate from MIPCM with the diffraction peak returning to the initial position. This new technology has potential application for detection of glucose and will also provide basis for the detection of other biomolecules
Template and target information: glucose


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