MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Mukherjee J, Gupta MN
Article Title: Dual bioimprinting of Thermomyces lanuginosus lipase for synthesis of biodiesel.
Publication date: 2016
Journal: Biotechnology Reports
Volume: 10
Page numbers: 38-43.
DOI: 10.1016/j.btre.2016.02.005
Alternative URL: http://www.sciencedirect.com/science/article/pii/S2215017X16300078

Abstract: Use of biodiesel as an alternative to non-renewable sources of energy has become an attractive option in recent years. The enzymatic synthesis of biodiesel by transesterification of fats/oils with an alcohol is a much more sustainable route than the chemical method. However, cost effectiveness of the enzymatic route is a major barrier in its commercialization. In this work, a high activity biocatalyst design of Thermomyces lanuginosus lipase is made by dually bioimprinting it with substrate and a surfactant (which is believed to open up the lid covering the active site of the lipase) during precipitation of the lipase in organic solvent. When the lipase was bioimprinted with only the surfactants, 28 U of the enzyme/g of oil could yield 99% biodiesel from soybean oil in about 4 h. However, when dually bioimprinted even very low enzyme load 1.4 U/g of oil, yielded 99% biodiesel within 48 h
Template and target information: bioimprinting, lipase
Author keywords: bioimprinting, lipases, transesterification, Enzyme precipitated and rinsed with organic solvents, Biodiesel, Biofuels, Enzymes in low water media


  Periodic table Prof shirt  Peptide peptide mug  Gravity waves shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner