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Abstract: A kind of molecular imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule. The results of Fourier transform infrared spectrometer (FT-IR) and scanning electron microscope showed that MIP had homogenous and uniform-sized cavities. It was confirmed that MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX1,4) than NIP according to the static equilibrium adsorption. The solid phase extraction columns were packed with MIP. The maximum recovery (85.05%) of MISPE was achieved using 0.1 mol/L acetic acid as washing solution and ethanol-water (95:5, V/V) as elution solution. Then the concentrations of GTX1,4 from culture solution of Alexandrium minutum and Alexandrium tamarense were determined to be 1.10 and 0.99 μg/L, respectively, under the optimum conditions of washing and elution, and RSD was 3.3% and 4.4%, respectively, indicated good LOD and higher precision.
Template and target information: guanosine, dummy template, gonyautoxins 1,4, GTX1,4
Author keywords: Gonyautoxins 1,4, molecularly imprinted solid phase extraction, Separation and preconcentration, Alexandrium minutum, Alexandrium tamarense