MIPdatabase
Authors: Umeno D, Kawasaki M, Maeda M
Publication date: 1998
Chapter title: Imprinting of proteins on polymer-coated DNA for affinity separation with enhanced selectivity.
Chapter number: 14
Page numbers: 202-216.
DOI: 10.1021/bk-1998-0703.ch014
Book title: Molecular and Ionic Recognition with Imprinted Polymers
Editors: Bartsch RA, Maeda M
Publisher: The American Chemical Society
City: Washington DC
Series title: ACS Symposium Series
Volume number: 703
Abstract: A temperature-responsive polymer, poly(N-isopropyl acrylamide) terminated with psoralen, has been synthesized. Photo-induced reaction between DNA and psoralen end group on the polymer resulted in the grafting of poly(N-isopropyl acrylamide) on double-stranded DNA. The conjugate was found to form a precipitate at temperatures above 31C and was easily collected by centrifugation. In this precipitation process, the conjugate collected DNA binders from solution. On the other hand, the binding efficiency of these molecules to the conjugate decreased significantly with an increasing level of DNA modification by the polymer. This blocking effect was utilized for protein imprinting on the DNA. Pre-incubation of the restriction endonuclease EcoRI with DNA results in selective preservation of its binding site from polymer modification. Bioimprinting is a potentially facile method for the preparation of selective collectors for site-specific DNA binders
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