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Reference type: Journal
Authors: Asliyuce S, Mattiasson B, Mamo G
Article Title: Synthesis and use of protein G imprinted cryogel as affinity matrix to purify protein G from cell lyaste.
Publication date: 2016
Journal: Journal of Chromatography B
Volume: 1021
Page numbers: 204-212.
DOI: 10.1016/j.jchromb.2015.12.060
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1570023215303846

Abstract: Monolithic macroporous cryogel imprinted with protein G was prepared using a functional co-monomer of N-methacryloyl-l-phenylalanine and 2-hydroxyethyl methacrylate. The chemical structure of the cryogel prepared was studied by FTIR-spectroscopy and its porosity was analysed using scanning electron microscopy. The cryogel was used to purify protein G from recombinant Escherichia coli cell lysate and the effect of pH, temperature, ionic strength, flow rate, etc on the adsorption of protein G to the monolithic column have been investigated. The selectivity of the imprinted cryogel was studied using protein A and myoglobin. It was possible to capture about 9 mg of Protein G per g of the cryogel
Template and target information: protein, protein G
Author keywords: Macroporous, antibodies, Molecular printing, Recombinant protein G


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