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Abstract: A selective molecularly imprinted polymer synthesized for the selective retention of cocaine (COC) and its metabolites [benzoylecgonine (BZE), ecgonine methyl ester (EME), and cocaethylene (CE)] was used as a solid adsorbent for assessing cocaine abuse by plasma analysis. The MIP beads (50 mg) were loaded inside a cone shaped device made of a polypropylene (PP) membrane for micro-solid-phase extraction (μ-SPE). High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for quantifying the analytes after MIP-μ-SPE. The best retention capabilities were reached when loading plasma samples (within the 0.1-5.0 mL range), previously adjusted to pH 5.5 by orbital-horizontal shaking (150 rpm, 50 -¦C) for 10 min. Analyte elution was achieved by subjecting the MIP-μ-SPE device to ultrasound (37 kHz, 325 W) with 10 mL of dichloromethane/2-propanol/ammonium hydroxide (76:20:4) for 8 min. After eluate evaporation to dryness and re-dissolution in 100 μL of mobile phase, the MIP-μ-SPE method yielded a pre-concentration factor of 50. Precision was assessed by intra-day and inter-day assays, and accuracy (intraday and inter-day analytical recovery, as well as the analysis of a BTMF 1/11-B control serum sample) show that the developed method is highly precise and accurate. In addition, the limits of detection, ranging from 0.061 ng mL-1 for COC to 0.87 ng mL-1 for BZE, were low enough for confirmative conclusions regarding cocaine abuse. The method was used for screening/quantifying cocaine and metabolites in plasma samples from poly-drug abusers
Template and target information: cocaine, COC, benzoylecgonine, BZE, ecgonine methyl ester, EME, cocaethylene, CE
Author keywords: Porous membrane-protected, molecularly imprinted polymer, Microsolid-phase extraction, plasma, Cocaine, High performance liquid chromatography tandem mass spectrometry