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Reference type: Journal
Authors: Zhang LL, Tang YH, Hao Y, He GY, Zhang BB, Gao RX, Zhang M
Article Title: Preparation of magnetic glycoprotein-imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white.
Publication date: 2016
Journal: Journal of Separation Science
Volume: 39
Issue: (10)
Page numbers: 1919-1925.
DOI: 10.1002/jssc.201600112

Abstract: Glycoproteins are crucial in massive physiological events and clinical application. It is necessary to prepare new materials to isolate the specific glycoprotein. New and simple core-shell molecularly imprinted polymers were prepared by surface imprinting. The polymers are synthesized with magnetic nanoparticles as the core, water-soluble dendritic polyethyleneimine as the monomer and the ovalbumin as the template. The prepared imprinted polymers showed thin imprinted shell, biocompatibility and superparamagnetic properties. The resultant materials exhibited fast kinetics, high adsorption capacity, perfect selectivity and reusability. More important, they can absorb the template glycoprotein from the neutral solution and successfully be applied to recognize the ovalbumin from egg white, which means that they can provide an alternate method to isolate glycoprotein from bodily fluids
Template and target information: protein, glycoprotein, ovalbumin
Author keywords: Dendritic polyethyleneimine, glycoproteins, magnetic separation, surface imprinting

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