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Reference type: Journal
Authors: Schwark S, Sun W, Stute J, Lutkemeyer D, Ulbricht M, Sellergren B
Article Title: Monoclonal antibody capture from cell culture supernatants using epitope imprinted macroporous membranes.
Publication date: 2016
Journal: RSC Advances
Volume: 6
Issue: (58)
Page numbers: 53162-53169.
DOI: 10.1039/C6RA06632A

Abstract: Epitope-imprinted membranes targeting the C-terminal fragment of the immunoglobuline G (IgG) heavy chain was developed and used for the purification of a commercial monoclonal antibody. The membranes exhibited strongly enhanced IgG affinity when compared with non-imprinted or IgG imprinted membranes reflected in binding selectivities in a protein mixture (IgG/HSA 1 : 10 w/w) of up to 40, and the elution of 95 to 100% pure IgG after washing. The dynamic binding capacity amounted to 3.9 mg mL-1 membrane volume with minor loss in performance upon repeated cleaning with alkali. The depletion of host cell proteins from a cell culture broth after production of anti-IL8 antibody using the best performing imprinted membrane under low-salt conditions reached 88% (0.7-1.2 log units) implying an effective removal of impurities from the cell culture supernatant
Template and target information: protein, peptide, C-terminal fragment of the immunoglobulin G, IgG

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