MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Prutthiwanasan B, Phechkrajang C, Suntornsuk L
Article Title: Fluorescent labelling of ciprofloxacin and norfloxacin and its application for residues analysis in surface water.
Publication date: 2016
Journal: Talanta
Volume: 159
Page numbers: 74-79.
DOI: 10.1016/j.talanta.2016.05.080
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914016304167

Abstract: Sensitivity enhancement for residue analysis of ciprofloxacin and norfloxacin in surface water was performed by liquid chromatography with fluorescent detection (LC-FD). Labelling of both drugs were studied with fluorescent probes (e.g. Nile blue perchlorate (NBP) and 4- (N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole (DBD-COCl). Factors affecting the derivatization (e.g. stoichiometric ratios, reaction time and base catalysts) were optimized. The derivatization was achieved in 15 min using a stoichiometric ratio between the substrate and DBD-COCl of 1:3, whereas NBP gave unsatisfactory results. Separation of the derivatives by LC was achieved (resolution (RS) > 1.8) on a C8 column using a mobile phase consisting of 50 mM formic acid and acetonitrile (ACN) (68:32% v/v) in 20 min. The method was linear (r2 > 0.99) in a range of 200-2,000 μg/L, precise (%RSD < 9.17) and accurate (%recovery of 102.5-122.2%) for the determination of the derivatives. The uses of fluoroquinolone molecularly imprinted polymer in conjunction with hydrophilic-lipophilic balance sorbents demonstrated an efficient procedure for sample pre-concentration and clean-up for water sample resulting in the improved percent recovery. Applications of the proposed method was shown in surface water samples in Thailand
Template and target information: ciprofloxacin, norfloxacin, commercial MIP
Author keywords: fluoroquinolone, Ciprofloxacin, norfloxacin, residue analysis, Fluorescent labelling


  Template of Doom spoof movie poster mug  Chemistry peptide mug  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner