Abstract: A piezoelectric molecularly imprinted polymeric sensor of a peptide, arg-gly-asp (RGD) is fabricated in this work. Gold-coated quartz crystals were used as piezoelectric platform for grafting RGDimprinted film. 3-mercaptopropionic acid was used to generate carboxyl groups on gold surface via thiol chemistry; which was activated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxylsuccinimide (NHS). RGD peptides were subsequently grafted on such activated carboxyl groups. Methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA) and azo-isobutyronitrile (AIBN) were used as functional monomer, crosslinker and initiator, respectively. On extraction of RGD peptides from thus grafted polymeric film, selective and sensitive receptor sites were generated on MIP-coated EQCM crystals. Hence, an electrochemical quartz crystal microbalance (EQCM)-MIP sensor with imprinting factor (IF) 2.27 for peptide was fabricated in a facile manner with detection limit of 18.5 ng mL-1 (3σ). Thus fabricated sensor was successfully applied in 'real' samples of blood plasma also.
Template and target information: peptide, arg-gly-asp, RGD
Author keywords: arg-gly-asp (RGD), Electrochemical quartz crystal microbalance (EQCM), molecularly imprinted polymer (MIP), peptide, piezoelectric sensor