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Reference type: Journal
Authors: Lian ZR, Li HB, Wang JT
Article Title: Experimental and computational studies on molecularly imprinted solid-phase extraction for gonyautoxins 2,3 from dinoflagellate Alexandrium minutum.
Publication date: 2016
Journal: Analytical and Bioanalytical Chemistry
Volume: 408
Issue: (20)
Page numbers: 5527-5535.
DOI: 10.1007/s00216-016-9644-3

Abstract: An innovative and effective extraction procedure based on molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation of gonyautoxins 2,3 (GTX2,3) from Alexandrium minutum sample. Molecularly imprinted polymer microspheres were prepared by suspension polymerization and and were employed as sorbents for the solid-phase extraction of GTX2,3. An off-line MISPE protocol was optimized. Subsequently, the extract samples from A. minutum were analyzed. The results showed that the interference matrices in the extract were obviously cleaned up by MISPE procedures. This outcome enabled the direct extraction of GTX2,3 in A. minutum samples with extraction efficiency as high as 83 %, rather significantly, without any need for a cleanup step prior to the extraction. Furthermore, computational approach also provided direct evidences of the high selective isolation of GTX2,3 from the microalgal extracts
Template and target information: gonyautoxins 2,3, GTX2,3
Author keywords: Gonyautoxins 2,3 Molecularly imprinted solid-phase extraction Alexandrium minutum Computational approach


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