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Reference type: Journal
Authors: Luo QH, Yu N, Shi CF, Wang XP, Wu JM
Article Title: Surface plasmon resonance sensor for antibiotics detection based on photo-initiated polymerization molecularly imprinted array.
Publication date: 2016
Journal: Talanta
Volume: 161
Page numbers: 797-803.
DOI: j.talanta.2016.09.049
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914016307196

Abstract: A surface plasmon resonance (SPR) sensor combined with nanoscale molecularly imprinted polymer (MIP) film as recognition element was developed for selective detection of the antibiotic ciprofloxacin (CIP). The MIP film on SPR sensor chip was prepared by in situ photo-initiated polymerization method which has the advantages of short polymerization time, controllable thickness and good uniformity. The surface wettability and thickness of MIP film on SPR sensor chip were characterized by static contact angle measurement and stylus profiler. The MIP-SPR sensor exhibited high selectivity, sensitivity and good stability for ciprofloxacin. The imprinting factors of the MIP-SPR sensor to ciprofloxacin and its structural analogue ofloxacin were 2.63 and 3.80, which is much higher than those to azithromycin, dopamine and penicillin. The SPR response had good linear relation with CIP concentration over the range 10-11 - 10-7 mol L-1. The MIP-SPR sensor also showed good repeatability and stability during cyclic detections. On the basis of the photo-initiated polymerization method, a surface plasmon resonance imaging (SPRi) chip modified with three types of MIP sensing spots was fabricated. The MIPs-SPRi sensor shows different response patterns to ciprofloxacin and azithromycin, revealing the ability to recognize different antibiotic molecules
Template and target information: ciprofloxacin, CIP
Author keywords: surface plasmon resonance, molecularly imprinted polymer, photo-initiated polymerization, surface plasmon resonance imaging, Antibiotics detection


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