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Authors: Yuan B, Yang XQ, Xue LW, Feng YN, Jiang JH
Article Title: A novel recycling system for nano-magnetic molecular imprinting immobilised cellulases: Synergistic recovery of anthocyanin from fruit and vegetable waste.
Publication date: 2016
Journal: Bioresource Technology
Volume: 222
Page numbers: 14-23.
DOI: 10.1016/j.biortech.2016.09.088
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0960852416313554
Abstract: Fruit and vegetable waste (FVW) is become a serious problem in developing countries. Enzymolysis is a potentially useful method for the treatment of FVW. In the present study, novel recycled magnetic molecular imprinting immobilised cellulases were prepared based on magnetic modified chitosan (MCTS) and Fe3O4. The properties of obtained were characterised by IR and grain-size measurements. Evaluation of a single factor affecting the loading efficiency of supports and the mixed immobilised enzymes showed better capacity than single immobilised, or free, enzymes. The immobilisation process could improve cellulase stability and repeatability of the method. Meanwhile, the kinetic parameters were also verified. The immobilised enzymes retained most of their capacity after 60 days' storage while free enzymes lost it within 30 days. Tests showed that the immobilised enzymes developed excellent capacity and five anthocyanins were collected
Template and target information: protein, cellulases
Author keywords: immobilisation, Nano-magnetic molecular imprinting, Fruit and vegetable waste, Synergistic recovery
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