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Reference type: Journal
Authors: Akiyama T, Hishiya T, Asanuma H, Komiyama M
Article Title: Molecular imprinting of cyclodextrin on silica-gel support for the stationary phase of high-performance-liquid-chromatography.
Publication date: 2001
Journal: Journal of Inclusion Phenomena and Macrocyclic Chemistry
Volume: 41
Issue: (1-4)
Page numbers: 149-153.
DOI: 10.1023/A:1014446420222

Abstract: By copolymerizing acryloyl cyclodextrin (CD) and N, N'- methylenebisacrylamide with vinylated silica-gel in the presence of a template in water, a thin layer of molecularly imprinted CD polymer was immobilized on a porous silica-gel support. This mechanically weak polymer was reinforced by the silica-gel and successfully used as a stationary phase in high- performance-liquid-chromatography (HPLC). When L-Phe-L-Phe was the template, the polymer/silica-gel conjugate retained L-Phe- L-Phe in the aqueous eluent more strongly than D-Phe-D-Phe. As expected, the D-Phe-D-Phe-imprinted polymer retained D-Phe-D- Phe more strongly than L-Phe-L-Phe. Selective separation of antibiotics was also achieved by the polymer/silica-gel conjugate. Molecularly imprinted CD polymer, immobilized on silica-gel, is an eminent stationary phase for HPLC in water
Template and target information: L-Phe-L-Phe, vancomycin


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