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Reference type: Journal
Authors: Zhang YN, Huang SM, Xu DD, Chen JS, Wu QZ, He JF
Article Title: Preparation of novel three-dimensionally ordered macroporous molecularly imprinted microspheres and its recognition for proteins.
Publication date: 2017
Journal: International Journal of Polymeric Materials and Polymeric Biomaterials
Volume: 66
Issue: (2)
Page numbers: 82-88.
DOI: 10.1080/00914037.2016.1182915

Abstract: A novel protein molecularly imprinted microsphere with three-dimensionally ordered macroporous (3DOM) structure has been successfully prepared with a combination of colloidal crystal template method and molecular imprinting technique by using bovine serum albumin as template, acrylamide as functional monomer, and N,N'-methylenebisacrylamide as crosslinker, respectively. SEM shows that the polymeric microspheres have a highly ordered three-dimensional interconnected macroporous structure with typical face-centered cubic lattice. The isothermal and kinetic adsorption behaviors of the polymer were investigated. Compared with the traditional bulk polymer, the 3DOM imprinted microspheres show higher binding rate constant, adsorption capability and specificity to template molecules
Template and target information: protein, bovine serum albumin, BSA
Author keywords: 3DOM, Binding properties, BSA, colloidal crystal, molecular imprinting

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