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Reference type: Journal
Authors: Chen J, Shinde S, Subedi P, Wierzbicka C, Sellergren B, Helling S, Marcus K
Article Title: Validation of molecularly imprinted polymers for side chain selective phosphopeptide enrichment.
Publication date: 2016
Journal: Journal of Chromatography A
Volume: 1471
Page numbers: 45-50.
DOI: 10.1016/j.chroma.2016.10.018
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967316313504

Abstract: Selective enrichment techniques are essential for mapping of protein posttranslational modifications (PTMs). Phosphorylation is one of the PTMs which continues to be associated with significant analytical challenges. Particularly problematic are tyrosine-phosphorylated peptides (pY-peptides) resulting from tryptic digestion which commonly escape current chemo- or immuno- affinity enrichments and hence remain undetected. We here report on significant improvements in this regard using pY selective molecularly imprinted polymers (pY-MIPs). The pY-MIP was compared with titanium dioxide (TiO2) affinity based enrichment and immunoprecipitation (IP) with respect to selective enrichment from a mixture of 13 standard peptides at different sample loads. At a low sample load (1 pmol of each peptide), IP resulted in enrichment of only a triply phosphorylated peptide whereas TiO2 enriched phosphopeptides irrespective of the amino acid side chain. However, with increased sample complexity, TiO2 failed to enrich the doubly phosphorylated peptides. This contrasted with the pY-MIP showing enrichment of all four tyrosine phosphorylated peptides at 1 pmol sample load of each peptide with a few other peptides binding unselectively. At an increased sample complexity consisting of the standard peptides spiked into mouse brain digest, the MIP showed clear enrichment of all four pY- peptides
Template and target information: tyrosine-phosphorylated peptides, pY-peptides
Author keywords: antibody, molecularly imprinted polymer, Phosphotyrosine, Phosphopeptide enrichment, TIO2

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