Abstract: A very cost-effective, fast, sensitive and specific imprinted polymer modified electrochemical sensor for the targeted detection, removal and destruction of Escherichia coli bacteria was developed onto the surface of Ag-ZnO bimetallic nanoparticle and graphene oxide nanocomposite. The nanocomposite played a dual role in this work, as a platform for imprinting of bacteria as well as a participated in their laser-light induced photo killing. In terms of sensing, our proposed sensor can detect E. Coli as few as 10 CFU mL-1 and capture 98% of bacterial cells from their very high concentrated solution (105 CFU mL-1). Similarly to the quantitative detection, we have also investigated the quantitative destruction of E. Coli and found that 16.0 cm2 area of polymer modified glass plate is sufficient enough to kill 105 CFU mL-1 in the small time span of 5 minutes. The obtained results suggest that our proposed sensor have potential to serve as a promising candidate for specific and quantitative detection, removal as well as the destruction of a variety of bacterial pathogens
Template and target information: Escherichia coli, bacteria, E. coli
Author keywords: Bacteria sensing, removal, Photothermal killing, imprinted polymer, GO-Ag@ZnO nanocomposite