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Reference type: Journal
Authors: Tirado-Guizar A, Paraguay-Delgado F, Pina-Luis GE
Article Title: A molecularly imprinted polymer-coated CdTe quantum dot nanocomposite for tryptophan recognition based on the Förster resonance energy transfer process.
Publication date: 2016
Journal: Methods and Applications in Fluorescence
Volume: 4
Issue: (4)
Page numbers: ArticleNo045003.
Alternative URL: http://stacks.iop.org/2050-6120/4/i=4/a=045003

Abstract: A new 'turn-on' Förster resonance energy transfer (FRET) nanosensor for l-tryptophan based on molecularly imprinted quantum dots (QDs) is proposed. The approach combines the advantages of the molecular imprinting technique, the fluorescent characteristics of the QDs and the energy transfer process. Silica-coated CdTe QDs were first synthesized and then molecularly imprinted using a sol-gel process without surfactants. The final composite presents stable fluorescence which increases with the addition of l-tryptophan. This 'turn-on' response is due to a FRET mechanism from the l-tryptophan as donor to the imprinted QD as acceptor. QDs are rarely applied as acceptors in FRET systems. The nanosensor shows selectivity towards l-tryptophan in the presence of other amino acids and interfering ions. The l-tryptophan nanosensor exhibits a linear range between 0 and 8 μM concentration, a detection limit of 350 nM and high selectivity. The proposed sensor was successfully applied for the detection of l-tryptophan in saliva. This novel sensor may offer an alternative approach to the design of a new generation of imprinted nanomaterials for the recognition of different analytes
Template and target information: l-tryptophan, tryptophan

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