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Reference type: Journal
Authors: García Mayor MA, Paniagua González G, Garcinuño Martínez RM, Fernández Hernando P, Durand Alegría JS
Article Title: Synthesis and characterization of a molecularly imprinted polymer for the determination of spiramycin in sheep milk.
Publication date: 2017
Journal: Food Chemistry
Volume: 221
Page numbers: 721-728.
DOI: 10.1016/j.foodchem.2016.11.114
Alternative URL: http://www.sciencedirect.com/science/article/pii/S030881461631963X

Abstract: A series of molecularly imprinted polymers (MIPs) comprising reactionary sites which are complementary to macrolide antibiotic spiramycin (SPI) were synthetized by noncovalent bulk polymerization technique. MIPs were synthesized under different polymerization process and their recognition efficiency was evaluated in binding studies in comparison with non-imprinted polymers. The best MIP was morphologically characterized and equilibrium assays were carried out. The MIP was evaluated as a sorbent for extraction and preconcentration of SPI from aqueous and sheep milk samples, and an off-line MISPE method followed by high-performance liquid chromatography with UV diode-array detection was established. Good linearity were obtained for SPI in a range of 24-965 μg kg-1 and the average recoveries at three spiked levels in milk samples were higher than 90% (RSD < 5%). Limit of quantification was 24.1 μg kg-1. Cross-reactivity studies from other macrolides with similar structure were tested. The optimum imprinted polymer showed a good selectivity and affinity for SPI, demonstrating the potential of the proposed MISPE for rapid, sensitive and effective sample pretreatment for selective determination of SPI in sheep milk samples
Template and target information: spiramycin, SPI
Author keywords: molecularly imprinted polymer, solid phase extraction, Spiramycin, Sheep milk


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